• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文OA文献 >The cAMP-dependent protein kinase catalytic subunit is required for appressorium formation and pathogenesis by the rice blast pathogen Magnaporthe grisea.
【2h】

The cAMP-dependent protein kinase catalytic subunit is required for appressorium formation and pathogenesis by the rice blast pathogen Magnaporthe grisea.

机译：稻瘟病病原菌Magnaporthe grisea需要cAMP依赖的蛋白激酶催化亚基来形成食欲和发病机理。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Magnaporthe grisea, the causal agent of rice blast disease, differentiates a specialized infection cell, an appressorium, that is required for infection of its host. Previously, cAMP was implicated in the endogenous signaling pathway leading to appressorium formation. To obtain direct evidence for the role of cAMP in appressorium formation, the gene encoding the catalytic subunit of the cAMP-dependent protein kinase (cpkA) was cloned, sequenced, and disrupted. Polymerase chain reaction primers designed after highly conserved regions in the same gene from other organisms were used to amplify genomic DNA fragments. The cloned amplification products were used to identify genomic clones. DNA blot analysis indicated that cpkA is present as a single copy in the genome. cpkA consists of 1894 bp, including three short introns sufficient to encode a protein of 539 amino acids with a predicted molecular mass of 60.7 kD. The deduced peptide shares > 45% identity with other catalytic subunits and contains all functional motifs and residues with the addition of a glutamine-rich region at the N terminus. Two transformants, L5 and T-182, in which cpkA had been replaced with a hygromycin resistance gene cassette, were unable to produce appressoria, could not be induced to form appressoria by cAMP, and were nonpathogenic on susceptible rice, even when leaves were abraded. These results were confirmed by analysis of 57 progeny from a cross between transformant L5 and the wild-type laboratory strain 70-6. Other aspects of growth and development, including vegetative growth as well as asexual and sexual competence, were unaffected when measured in vitro. These results provide direct evidence that the cAMP-dependent protein kinase is necessary for infection-related morphogenesis and pathogenesis in a phytopathogenic fungus.
机译：稻瘟病的病原体稻瘟病菌（Magnaporthe grisea）分化出感染宿主所需的专门感染细胞，即食欲细胞。以前，cAMP参与导致内脏形成的内源性信号传导途径。为了获得cAMP在前肢形成中的作用的直接证据，克隆了编码cAMP依赖性蛋白激酶（cpkA）催化亚基的基因，进行了测序和破坏。根据来自其他生物的同一基因中高度保守的区域设计的聚合酶链反应引物可用于扩增基因组DNA片段。克隆的扩增产物用于鉴定基因组克隆。 DNA印迹分析表明cpkA在基因组中以单拷贝形式存在。 cpkA由1894 bp组成，包括三个短内含子，足以编码539个氨基酸的蛋白质，预测分子量为60.7 kD。推导的肽与其他催化亚基共享> 45％的同一性，并包含所有功能性基序和残基，并在N端添加了富含谷氨酰胺的区域。 L5和T-182这两个转化子，其中的cpkA被潮霉素抗性基因盒替代，不能产生束生无性，不能被cAMP诱导形成束生性，即使在叶片磨损的情况下，对易感水稻也没有致病性。通过分析来自转化体L5和野生型实验室菌株70-6之间的杂交的57个后代，证实了这些结果。体外测量不影响生长发育的其他方面，包括营养生长以及无性和性能力。这些结果提供了直接的证据，证明依赖cAMP的蛋白激酶对于植物致病真菌中与感染相关的形态发生和发病机理是必需的。

著录项

  • 作者

    Mitchell, T K; Dean, R A;

  • 作者单位
  • 年度 1995
  • 总页数
  • 原文格式 PDF
  • 正文语种 en
  • 中图分类

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号